Journal: CytoJournal

Article Title: Combine immune checkpoint indoleamine 2,3-dioxygenase 1 knockdown with ferroptosis inducer Erastin/RSL3 accelerates colorectal cancer cell ferroptosis

doi: 10.25259/Cytojournal_262_2024

Figure Lengend Snippet: Effect of Erastin and RSL3 on IDO1 expression in CRC cells and normal colonic epithelial cell lines. (a) RT-qPCR was used to detect the mRNA expression of IDO1 in MC38, CT26, HT29, and NCM460 cells. (b) Western blot analysis was performed to analyze the protein levels of IDO1 in MC38, CT26, HT29, and NCM460 cells. The mRNA and protein expressions of the MOCK group were normalized to 1. Data is reported as mean ± SD with three replicates. n = 3. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001, ns: No significance. IDO1: Immune checkpoint indoleamine 2,3-dioxygenase 1, CRC: Colorectal cancer, RT-qPCR: Reverse transcription quantitative polymerase chain reaction, SD: Standard deviation, mRNA: Messenger RNA.

Article Snippet: All RT-qPCR primers [ ] were custom designed and synthesized by Sangon Biotech (China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: CytoJournal

Article Title: Combine immune checkpoint indoleamine 2,3-dioxygenase 1 knockdown with ferroptosis inducer Erastin/RSL3 accelerates colorectal cancer cell ferroptosis

doi: 10.25259/Cytojournal_262_2024

Figure Lengend Snippet: Construction of IDO1 knockdown in MC38 cell line and validation of expression levels of ferroptosis-related genes. (a) Western blot was used to detect IDO1 knockdown at the protein level. (b) RT-qPCR was used to detect IDO1 knockdown at the mRNA level. (c) Western blot analysis was performed to analyze the protein levels of GPX4, SLC7A11, COX-2, ACSL4, NOX1, NRF2, and FTH1 in MC38-sh-IDO1 and MC38-sh-NC. (d) RT-qPCR was performed to analyze the mRNA levels of GPX4, SLC7A11, COX-2, ACSL4, NOX1, NRF2, and FTH1 in MC38-sh-IDO1 and MC38-sh-NC. Data is reported as mean ± SD with three replicates. n = 3. ✶ P < 0.05, ✶ ✶ P < 0.01, ✶ ✶ ✶ P < 0.001. IDO1: Immune checkpoint indoleamine 2, 3-dioxygenase 1, GPX4: Glutathione peroxidase 4, SLC7A11: Solute carrier family 7 member 11, COX-2: Cyclooxygenase-2, ACSL4: Acyl-CoA synthetase long-chain family member 4, NOX1: NADPH oxidase 1, NRF2: Nuclear factor E2-related factor 2, FTH1: Ferritin heavy polypeptide 1, RT-qPCR: Reverse transcription quantitative polymerase chain reaction, SD: Standard deviation, mRNA: Messenger RNA, GAPDH: Glyceraldehyde-3-phosphate dehydrogenase, sh-NC: short hairpin-negative control.

Article Snippet: All RT-qPCR primers [ ] were custom designed and synthesized by Sangon Biotech (China).

Techniques: Knockdown, Biomarker Discovery, Expressing, Western Blot, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation, Negative Control

Journal: iScience

Article Title: MiR-1202 increases radioresistance in nasopharyngeal carcinoma by targeting NAIF1/MAPK/ERK pathway

doi: 10.1016/j.isci.2026.115135

Figure Lengend Snippet: miR-1202 overexpression is associated with a radioresistant phenotype of NPC in vitro (A) The expression of miR-1202 in CNE-2, C666-1, and CNE-2R cells was detected by RT-qPCR. The U6 gene was used as housekeeping gene. Samples were normalized to the CNE-2R cultures. (B) CNE-2 and C666-1 cell lines were transfected with either a control vector (mimic control) or a miR-1202 overexpression vector (miR-1202 mimic). CNE-2R cell line was transfected with either a control vector (inhibitor control) or a miR-1202 silence vector (miR-1202 inhibitor). RT-qPCR was used to measure miR-1202 expression post-transfection. The U6 gene was used as housekeeping gene for RT-qPCR analysis (mean ± SD; n = 3; t test; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0. 001, ∗∗∗∗ p < 0. 0001).

Article Snippet: Primers for RT-qPCR, see , Sangon Biotech , –.

Techniques: Over Expression, In Vitro, Expressing, Quantitative RT-PCR, Transfection, Control, Plasmid Preparation

Journal: iScience

Article Title: MiR-1202 increases radioresistance in nasopharyngeal carcinoma by targeting NAIF1/MAPK/ERK pathway

doi: 10.1016/j.isci.2026.115135

Figure Lengend Snippet: NAIF1 increases radiosensitivity of NPC in vitro (A and B) The mRNA expression of NAIF1 in CNE-2 and C666-1 cells after transfection was detected by RT-qPCR. The GAPDH gene was used as housekeeping gene. (C and D) The protein expression of NAIF1 in CNE-2 and C666-1 cells after transfection was detected by Western blot. (E and F) The survival fraction of NAIF1-knockdown CNE-2 and C666-1 cells and their control cells after 0, 2, 4, 6, and 8 Gy of X-ray IR was measured by CCK-8 assay. (G and H) The colony formation ability of the NAIF1-knockdown CNE-2 and C666-1 cells and their control cells after 0, 2, 4, 6, and 8 Gy of X-ray IR was measured by colony-forming assay; and the dose-survival curves were calculated and fitted to a single-hit multi-target model (mean ± SD; n = 3; t test; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001).

Article Snippet: Primers for RT-qPCR, see , Sangon Biotech , –.

Techniques: In Vitro, Expressing, Transfection, Quantitative RT-PCR, Western Blot, Knockdown, Control, CCK-8 Assay